| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.161843 |
| Chromosome: | chromosome 8 |
| Location: | 1207067 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g363150 | (1 of 2) PTHR23301//PTHR23301:SF0 - CHITIN BINDING PERITROPHIN-A // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAACTTGCAGCGCGTTGCTTGGTTGGT |
| Internal bar code: | GCCGATACGGGGACCGTGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 438 |
| LEAP-Seq percent confirming: | 99.8958 |
| LEAP-Seq n confirming: | 1917 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACCTGCAGCACTGTCAT |
| Suggested primer 2: | CGAGCGAGTTCATAGCATCA |