Insertion junction: LMJ.RY0402.161846_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.161846
Chromosome:chromosome 12
Location:7509029
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g555950 DHC11 Axonemal Dynein Heavy Chain 11; (1 of 1) PTHR10676//PTHR10676:SF265 - DYNEIN HEAVY CHAIN FAMILY PROTEIN // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTGTTCCGTGACGTGGCCATGATGGTGC
Internal bar code:GCGCTGCGCAGGTCACACCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:211
LEAP-Seq percent confirming:30.0728
LEAP-Seq n confirming:578
LEAP-Seq n nonconfirming:1344
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGCCTAGACTACCTGGCT
Suggested primer 2:ACAGGATGCCCTTGAACAAC