Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.161846 |
Chromosome: | chromosome_12 |
Location: | 7509055 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g555950 | DHC11 | Putative flagellar inner arm dynein heavy chain, novel minor typ | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGGTTGGAAAGACGTGAGGGAGCGAACGCA |
Internal bar code: | ACACATGCAAACCAGGCGTTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 881 |
LEAP-Seq percent confirming: | 96.9869 |
LEAP-Seq n confirming: | 2221 |
LEAP-Seq n nonconfirming: | 69 |
LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACGGCCTAGACTACCTGGCT |
Suggested primer 2: | ACAGGATGCCCTTGAACAAC |