| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161875 |
| Chromosome: | chromosome_6 |
| Location: | 2880854 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g272050 | GPM1,PGM1 | Phosphoglycerate mutase, 2,3-bisphosphoglycerate-independent | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGACTATAATTGCCGTGAGGAACAATGGG |
| Internal bar code: | TGGGAAATGAACAATTCGTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 335 |
| LEAP-Seq percent confirming: | 99.8084 |
| LEAP-Seq n confirming: | 2604 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAATTCCCAGATCCAGTA |
| Suggested primer 2: | GGAATGGCAGAAACTGTGGT |