| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161890 |
| Chromosome: | chromosome 1 |
| Location: | 2841984 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g017100 | (1 of 1) IPR002123//IPR029058 - Phospholipid/glycerol acyltransferase // Alpha/Beta hydrolase fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGTGTGATGTGAGAGAGTGATGCGTAG |
| Internal bar code: | GACACCCGGGGGAAAAGCACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 648 |
| LEAP-Seq percent confirming: | 99.2219 |
| LEAP-Seq n confirming: | 7269 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAGCGGGATCAGCAGTAG |
| Suggested primer 2: | TTCTTCACCACCTCCCTGAC |