| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161893 |
| Chromosome: | chromosome 6 |
| Location: | 3468653 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278095 | (1 of 1) IPR016024//IPR027417 - Armadillo-type fold // P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGTGGCTTGCACCGTGGTGATCAGTAT |
| Internal bar code: | GTGTTTCCCAGAGCGTTGAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 812 |
| LEAP-Seq percent confirming: | 99.7041 |
| LEAP-Seq n confirming: | 7751 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGAGCATTAAGCAGCTCG |
| Suggested primer 2: | CCAGCTCCCAGCTTTGATAG |