Insertion junction: LMJ.RY0402.161899_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.161899
Chromosome:chromosome 10
Location:1312951
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g427550 (1 of 3) IPR022751//IPR029044 - Alpha-mannosyltransferase // Nucleotide-diphospho-sugar transferases 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTGTCAGGGATGTGTACGATGTTTGGTG
Internal bar code:GTAGTAACTAGGCATATGAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:811
LEAP-Seq percent confirming:99.4822
LEAP-Seq n confirming:1729
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCGACACCTTCAAAAAGC
Suggested primer 2:TCAGGTGCTGTCTTGGACTG