| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161906 |
| Chromosome: | chromosome 10 |
| Location: | 1583899 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429300 | (1 of 2) IPR001680//IPR001810//IPR017986 - WD40 repeat // F-box domain // WD40-repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTTCATGCCATCCTCTCGCGGCTGGACC |
| Internal bar code: | AGGATGGTATTTCTGCTTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 464 |
| LEAP-Seq percent confirming: | 83.391 |
| LEAP-Seq n confirming: | 482 |
| LEAP-Seq n nonconfirming: | 96 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAAGCAGCGAGGAATTCAA |
| Suggested primer 2: | GCAGGAGTTCCTGTTTCTGC |