| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161910 |
| Chromosome: | chromosome 13 |
| Location: | 238143 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g563700 | FAP369 | Flagellar Associated Protein 369; (1 of 1) IPR001680//IPR011047//IPR017986//IPR027417 - WD40 repeat // Quinonprotein alcohol dehydrogenase-like superfamily // WD40-repeat-containing domain // P-loop containing nucleoside triphosphate hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTAGGCTGGAGGGCAGCTCGCTCAGAGA |
| Internal bar code: | TGTTCAGAGAGCATCCGCGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 212 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGGAGTCACTGACGGTT |
| Suggested primer 2: | AAGCGTACGTCGTGTGAGTG |