| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161911 |
| Chromosome: | chromosome 1 |
| Location: | 4244160 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028423 | (1 of 1) 4.1.1.25 - Tyrosine decarboxylase / L-tyrosine carboxy-lyase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGACCCCCAAACCCCGACACGCCCCCG |
| Internal bar code: | GGTGGATGGCCGGCGTGCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 445 |
| LEAP-Seq percent confirming: | 79.3651 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCCAAACAGACCGAGAT |
| Suggested primer 2: | GACCAAGTTGTGATGCCCTT |