| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.161931 |
| Chromosome: | chromosome 12 |
| Location: | 6054287 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535350 | (1 of 1) IPR011044//IPR017986 - Quinoprotein amine dehydrogenase, beta chain-like // WD40-repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCTGAGGGGGGGCGGTTACATTCATGA |
| Internal bar code: | TGTTTGAGTTTGCCCGACCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 257 |
| LEAP-Seq percent confirming: | 98.009 |
| LEAP-Seq n confirming: | 1083 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCACACCTCTCTTCCCAC |
| Suggested primer 2: | GGAGATAAAGAGCTGGCGTG |