| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.161933 |
| Chromosome: | chromosome 4 |
| Location: | 4082968 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g233302 | (1 of 2) PTHR23132//PTHR23132:SF9 - D-ALANINE--D-ALANINE LIGASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCACCGCAAGCTGCGCGTTTGCGTGCTT |
| Internal bar code: | CCTCCATACCTCTGGGGACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1101 |
| LEAP-Seq percent confirming: | 98.7737 |
| LEAP-Seq n confirming: | 29480 |
| LEAP-Seq n nonconfirming: | 366 |
| LEAP-Seq n unique pos: | 144 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTCTCAATTCTCGCAGGC |
| Suggested primer 2: | AGTGGCTGTGCTTCATGTTG |