| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161937 |
| Chromosome: | chromosome 2 |
| Location: | 2005987 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g088450 | CPLD29 | Conserved in the Plant Lineage and Diatoms; (1 of 1) PTHR22999:SF18 - PX DOMAIN-CONTAINING PROTEIN KINASE-LIKE PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGTGAAGTGCCATCAGCGCCATACCTTA |
| Internal bar code: | TTCAGTACAATAGTTGAGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 99.7212 |
| LEAP-Seq n confirming: | 1073 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAAGCACAAAGCATGCAG |
| Suggested primer 2: | CTTCGGAATTCCTGGTTTGA |