| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.161963 |
| Chromosome: | chromosome 4 |
| Location: | 2793637 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223900 | (1 of 2) K09313 - homeobox protein cut-like (CUTL) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCACAGTCGCAGCAAAGTCATAATCACAG |
| Internal bar code: | AATTACAACGCTGGAAGGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 709 |
| LEAP-Seq percent confirming: | 99.6839 |
| LEAP-Seq n confirming: | 2523 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCACACACACACACACAC |
| Suggested primer 2: | CCTTCCTGCTGTCTACCTCG |