| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.161964 |
| Chromosome: | chromosome 16 |
| Location: | 6005491 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676500 | (1 of 1) PTHR17630//PTHR17630:SF54 - DIENELACTONE HYDROLASE // ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGGGCGGCGCGTGGGCTATCGGCGGCTT |
| Internal bar code: | ACCCGCCCGTGTTCTAGTTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 560 |
| LEAP-Seq percent confirming: | 99.8826 |
| LEAP-Seq n confirming: | 851 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAATGTGTATGAGATGGCG |
| Suggested primer 2: | CTCACCCGGTAGCAGTTTGT |