| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161968 |
| Chromosome: | chromosome 2 |
| Location: | 5574901 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108900 | CAM6 | Calmodulin-like protein; (1 of 140) IPR002048 - EF-hand domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATATATTCTGGGCCGTAGTGCTCAGGTA |
| Internal bar code: | CTACGTGGATTTTAGCAAAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 945 |
| LEAP-Seq percent confirming: | 99.5484 |
| LEAP-Seq n confirming: | 6613 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTGCCGCCTAGTTCAAGT |
| Suggested primer 2: | CAGCAACACCTTGCGTTCTA |