| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161981 |
| Chromosome: | chromosome 12 |
| Location: | 8454843 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548200 | (1 of 1) PTHR21004:SF0 - PEROXISOMAL LEADER PEPTIDE-PROCESSING PROTEASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTTTGCATAATGAAATTACACTACGGTA |
| Internal bar code: | GCTTAGTCGGGGCGTCAATCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 594 |
| LEAP-Seq percent confirming: | 99.3234 |
| LEAP-Seq n confirming: | 5578 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACTACACCGCCAGTATG |
| Suggested primer 2: | CAACCATAGTGTGCCAGGTG |