Insertion junction: LMJ.RY0402.162002_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.162002
Chromosome:chromosome 7
Location:2187926
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g326950 (1 of 2) K15437 - aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (AIMP1, ARC1) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGTGGAAATAGCGATCTGACTCAAGTTG
Internal bar code:CGCCCTTTCTCGTCAGACTAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:320
LEAP-Seq percent confirming:99.4413
LEAP-Seq n confirming:712
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTACACACGATCCGACCCTT
Suggested primer 2:CTAACCTCCCCTTCCCTCAC