| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162041 |
| Chromosome: | chromosome 6 |
| Location: | 8338751 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g306350 | FDX3 | Apoferredoxin; (1 of 2) PTHR19370//PTHR19370:SF110 - NADH-CYTOCHROME B5 REDUCTASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCATGGGTGAACGTATGCGTGCGCGTGTG |
| Internal bar code: | GTGCGACCGCGGGACCGGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 635 |
| LEAP-Seq percent confirming: | 99.3834 |
| LEAP-Seq n confirming: | 1773 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAAAGAAGGACAAGTCGC |
| Suggested primer 2: | TCGTGAGTCTTGTGACCTGC |