| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162052 |
| Chromosome: | chromosome 13 |
| Location: | 2158110 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578100 | NUP205 | (1 of 1) K14310 - nuclear pore complex protein Nup205 (NUP205, NUP192); Nucleoporin 205 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGCAGGTGCGTGGGTTGGCCTGGAGGA |
| Internal bar code: | GGTAGGATAGGTGAGCGGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 102 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 139 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTCGTCTGCTGTTGTGAC |
| Suggested primer 2: | GAAACAGGTTGACGGGAAAA |