| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.162062 |
| Chromosome: | chromosome 3 |
| Location: | 8253719 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199450 | MINE2 | Chloroplast division site determinant | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTGTATTACTTGACTGTAACTGCTTATT |
| Internal bar code: | ACCAGTAGCATCGGCGCATTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 947 |
| LEAP-Seq percent confirming: | 99.6169 |
| LEAP-Seq n confirming: | 2080 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATGTGCGTCTGTGCGTCT |
| Suggested primer 2: | CGGGGGTGATAATTTGAATG |