| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162066 |
| Chromosome: | chromosome 8 |
| Location: | 668961 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g360050 | DUR1 | Urea carboxylase/allophanate hydrolase; (1 of 1) 6.3.4.6 - Urea carboxylase / Urease (ATP-hydrolyzing) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCGGAGTTATAGGGGGTCAGGATGTGG |
| Internal bar code: | TGCGCTTCTGAGGTGGGGTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 422 |
| LEAP-Seq percent confirming: | 99.8065 |
| LEAP-Seq n confirming: | 2063 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTAAAACACGAGCGAAA |
| Suggested primer 2: | TTGCGTGATTACTGCTTTGC |