| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162125 |
| Chromosome: | chromosome 16 |
| Location: | 3498355 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675246 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGTGATCAGTAGCCAGGCACACACAAA |
| Internal bar code: | CCTGGGCACACAGGACAGCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 657 |
| LEAP-Seq percent confirming: | 99.8358 |
| LEAP-Seq n confirming: | 1216 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTACTTCGTCGTCCTCC |
| Suggested primer 2: | CAACGTGTCACGGTATGAGG |