Insertion junction: LMJ.RY0402.162128_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.162128
Chromosome:chromosome 3
Location:5367287
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g184400 (1 of 3) 3.6.1.22//3.6.1.55 - NAD(+) diphosphatase / NADP pyrophosphatase // 8-oxo-dGTP diphosphatase / 8-oxo-dGTPase; Putative NAD+ diphosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGTATGCTGGGAGGTCCTACCCGAGGCG
Internal bar code:TATTGCAATTTGTCTGCTCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:749
LEAP-Seq percent confirming:98.6413
LEAP-Seq n confirming:363
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCCTCTGCCGTACTCTCGTT
Suggested primer 2:GTCGCGGTTGTATCCCTTTA