| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.162134 |
| Chromosome: | chromosome 12 |
| Location: | 4042359 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517300 | (1 of 1) PF07738 - Sad1 / UNC-like C-terminal (Sad1_UNC) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGGGCCAAACGCTGCGTGTTTGTTACA |
| Internal bar code: | GGAGTCCCGATACTGTGGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 99.5496 |
| LEAP-Seq n confirming: | 1105 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTATTGTTGAGCCCTGC |
| Suggested primer 2: | GGAGCAGCGCCTAGTATGAC |