| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.162202 |
| Chromosome: | chromosome 8 |
| Location: | 266299 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358557 | (1 of 2) PF10173 - Mitochondrial K+-H+ exchange-related (Mit_KHE1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTGGGGCCGAGGCTAGCTCGAGGCTTT |
| Internal bar code: | ACCGTGTCTTCGCATGCTGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 273 |
| LEAP-Seq percent confirming: | 99.5763 |
| LEAP-Seq n confirming: | 235 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGTGGTGGTGGAATGGT |
| Suggested primer 2: | TGCTCACCTTGAGTGCAAAC |