| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162202 |
| Chromosome: | chromosome 8 |
| Location: | 266756 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358557 | (1 of 2) PF10173 - Mitochondrial K+-H+ exchange-related (Mit_KHE1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCTGCTTCATTGCGCTATTTACGGTGTA |
| Internal bar code: | GAGCAAAGCACGAGGCAGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1013 |
| LEAP-Seq percent confirming: | 99.8988 |
| LEAP-Seq n confirming: | 2962 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTTCCATTGCTTTTGTGT |
| Suggested primer 2: | ACCGCCCAAGCTTCTAAAAT |