| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162224 |
| Chromosome: | chromosome 7 |
| Location: | 5303722 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g349520 | (1 of 1) PF00023//PF00069//PF13857 - Ankyrin repeat (Ank) // Protein kinase domain (Pkinase) // Ankyrin repeats (many copies) (Ank_5) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGAATGCTATGGAAAGGTCGGCTGTATG |
| Internal bar code: | GTGTTAAGTAAGGCGCCCCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 753 |
| LEAP-Seq percent confirming: | 98.8809 |
| LEAP-Seq n confirming: | 3446 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTTGGGATCCTGAACAGA |
| Suggested primer 2: | TGTCCTTCTGCAAGCATTTG |