| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162237 |
| Chromosome: | chromosome 1 |
| Location: | 2012266 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010832 | (1 of 1) PTHR12411:SF389 - CATHEPSIN L1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAAGTGGGACCTTTTGTGACTCTGTGTA |
| Internal bar code: | ACGGTAGTCGCAGCGCATTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 775 |
| LEAP-Seq percent confirming: | 99.6044 |
| LEAP-Seq n confirming: | 2014 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATGCCACAGCTACTGCT |
| Suggested primer 2: | AGGCTGATCATGGGTAGGTG |