| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.162237 |
| Chromosome: | chromosome 1 |
| Location: | 7872634 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g060712 | (1 of 1) IPR002867//IPR031127 - IBR domain // E3 ubiquitin ligase RBR family | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCACCTGTAGTGGGTCAGTGCGAGGCGGG |
| Internal bar code: | CTGCGCTTTCCCGGCCGTTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 178 |
| LEAP-Seq percent confirming: | 99.5602 |
| LEAP-Seq n confirming: | 1811 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGGCAGGAGTGAAATACT |
| Suggested primer 2: | GGCTGAAGACTCGGAGTACG |