| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.162262 |
| Chromosome: | chromosome 10 |
| Location: | 6055269 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g463300 | (1 of 1) PTHR31515:SF0 - TRANSMEMBRANE PROTEIN | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAATCAAGTCCTTCTGTACAAGCCATGAT |
| Internal bar code: | TGCGGAGGAGCTTTACGCACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 462 |
| LEAP-Seq percent confirming: | 99.5776 |
| LEAP-Seq n confirming: | 3065 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACCATTGGCCTCCTTAT |
| Suggested primer 2: | TGTGTGTGTGTATGTTGCGG |