| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.162264 |
| Chromosome: | chromosome 10 |
| Location: | 1588342 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429350 | (1 of 6) 3.4.22.69 - SARS coronavirus main proteinase / Severe acute respiratory syndrome coronavirus main protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCTCTCGGTAGCGCTCTCACCAGCACA |
| Internal bar code: | TTCGTGGCGTGTAATTTATCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 538 |
| LEAP-Seq percent confirming: | 60.9756 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCTGACCCTTCCTACTG |
| Suggested primer 2: | TGACTGGGTGAGTGAGTGGA |