Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.162279 |
Chromosome: | chromosome 11 |
Location: | 3030002 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g478650 | (1 of 6) IPR007577//IPR029044 - Glycosyltransferase, DXD sugar-binding motif // Nucleotide-diphospho-sugar transferases | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAAACGGGGGGGAGCAGGTGGGGGGCGG |
Internal bar code: | ACGGTTAGCAAGTCGAAGGACG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 298 |
LEAP-Seq percent confirming: | 54.0541 |
LEAP-Seq n confirming: | 20 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTTGGTTTAGTTTGGGCTGG |
Suggested primer 2: | CGTCGGCACTTCACTTCATA |