| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162280 |
| Chromosome: | chromosome 8 |
| Location: | 9685 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358200 | ACV4 | protein kinase; (1 of 6) PTHR23257//PTHR23257:SF529 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGGTGCGGGTGGGCGGCGACCGCAGTG |
| Internal bar code: | CGCGTAAGGTGGAACGGGCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 47 |
| LEAP-Seq percent confirming: | 60.9375 |
| LEAP-Seq n confirming: | 195 |
| LEAP-Seq n nonconfirming: | 125 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGCCTCAACTACGACAC |
| Suggested primer 2: | AGTCGCTCCACACGAGACTT |