| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.162308 |
| Chromosome: | chromosome 3 |
| Location: | 644010 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145807 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCAAACGCCTGTCGCCTGCCTGTTTGAT |
| Internal bar code: | TGTCGTGCCGACCTACTAACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 361 |
| LEAP-Seq percent confirming: | 99.6207 |
| LEAP-Seq n confirming: | 1576 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCCAGCAACAGCTATAAA |
| Suggested primer 2: | TTCCGTTGCAATTTCTGTTG |