Insertion junction: LMJ.RY0402.162321_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.162321
Chromosome:chromosome 12
Location:787024
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g495701 (1 of 20) IPR001190//IPR017448 - SRCR domain // SRCR-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGACTGACACGCTGCCAGAGTCGCTATG
Internal bar code:AGTAGGGGTGGCGCGACCGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:40
LEAP-Seq percent confirming:99.4475
LEAP-Seq n confirming:180
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGAGTAGACGCTTGAAAC
Suggested primer 2:CTTGTTGGTGTTTGTGACGG