| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162345 |
| Chromosome: | chromosome 4 |
| Location: | 3139475 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225950 | (1 of 11) PF00439 - Bromodomain (Bromodomain) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAGATGGGCGTCCTGACTGGGTTTACCG |
| Internal bar code: | GGGTTCGCAGGTGCAAGCGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 99.0926 |
| LEAP-Seq n confirming: | 3276 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTATCTAGACGAGGACG |
| Suggested primer 2: | GAGACTGCTGAGTGATGCCA |