| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162373 |
| Chromosome: | chromosome 1 |
| Location: | 5483822 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038600 | FAD7 | Omega-3-fatty acid desaturase; (1 of 1) K10257 - omega-3 fatty acid desaturase (delta-15 desaturase) (FAD8, desB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAAGAGAACTAAACACCATGCAGACCCA |
| Internal bar code: | AAGCGGGAAGTCGCATGGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 929 |
| LEAP-Seq percent confirming: | 98.5379 |
| LEAP-Seq n confirming: | 3572 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCGTTATGGTAGTCCATC |
| Suggested primer 2: | CTCCACACTGCTTTGAACGA |