Insertion junction: LMJ.RY0402.162377_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.162377
Chromosome:chromosome 6
Location:6310908
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g291750 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAACTCAGGAAGGGACCGTCCAATGAGGG
Internal bar code:CACCCTCAGAGTAAATCGCAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1092
LEAP-Seq percent confirming:95.5267
LEAP-Seq n confirming:662
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGCCTAAGGTTGTCGAGC
Suggested primer 2:CAGAAGGATTTGTTGGGGAA