Insertion junction: LMJ.RY0402.162411_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.162411
Chromosome:chromosome 2
Location:282484
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g075050 PTA1 (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain; Proton/phosphate symporter CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAACAAGGTGCGCGGTGGGGAAGTATGC
Internal bar code:AGCTAGGCAATTACGCGAAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:692
LEAP-Seq percent confirming:99.3218
LEAP-Seq n confirming:2636
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TACCGCTTTTCCAAACATCC
Suggested primer 2:ACTGACCTGGAAACCCAGTG