| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162422 |
| Chromosome: | chromosome 1 |
| Location: | 7244795 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g052200 | (1 of 1) PF12923 - Ribosomal RNA-processing protein 7 (RRP7) (RRP7) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCGACAGACAGTACATCGTAGTCCTCC |
| Internal bar code: | ACGTCAGAGAGGTCGCGGCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 786 |
| LEAP-Seq percent confirming: | 99.3284 |
| LEAP-Seq n confirming: | 2810 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGTCAAAGACGCACAAGG |
| Suggested primer 2: | CTCCCTGAATCTCCAAACCA |