| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.162425 |
| Chromosome: | chromosome 2 |
| Location: | 6961681 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g120350 | (1 of 100) IPR001810 - F-box domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGGCCGCGGTCGTAGTGCGGGTGCAGC |
| Internal bar code: | GGACCTAGTTAGCGGAGGAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 984 |
| LEAP-Seq percent confirming: | 99.5798 |
| LEAP-Seq n confirming: | 948 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCCTCTTCCTCCTCGT |
| Suggested primer 2: | AGGAGCCATTTCAAAGAGCA |