| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.162428 |
| Chromosome: | chromosome 6 |
| Location: | 836409 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g254450 | (1 of 1) K10295 - F-box protein 9 (FBXO9) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGTCACGCCCCGCAGCCCCCAGATACTG |
| Internal bar code: | AGGACACACTCGATACCCGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 129 |
| LEAP-Seq percent confirming: | 98.0392 |
| LEAP-Seq n confirming: | 150 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCATCTTACAACCCACTT |
| Suggested primer 2: | GTTGTGGCCAGACCTTTTGT |