Insertion junction: LMJ.RY0402.162432_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.162432
Chromosome:chromosome 9
Location:4747288
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g397179 OPR13 OctotricoPeptide Repeat protein 13 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCAGACACATACACACACACGCACACAC
Internal bar code:GGGACGACCTGCTATCGGACAA

Confirmation - LEAP-Seq

LEAP-Seq distance:642
LEAP-Seq percent confirming:65.2379
LEAP-Seq n confirming:3181
LEAP-Seq n nonconfirming:1695
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGTAAGGTGGGGGTAGGC
Suggested primer 2:GAAGGCGTAGCCAGGTACAG