| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.162444 |
| Chromosome: | chromosome 9 |
| Location: | 2413301 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391600 | MEP1 | Putative IgA-specific metalloendopeptidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGGCCATGCCCGGTGTTATCTACCACAG |
| Internal bar code: | AGTCGTCTCCGGTCTACCAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 525 |
| LEAP-Seq percent confirming: | 57.6271 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACAGCACCACCAGAACA |
| Suggested primer 2: | ACTCCAGCATCGCACTGTC |