| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.162481 |
| Chromosome: | chromosome_2 |
| Location: | 4262111 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g098900 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGACGTCACATTGGGGCTGGCCGTAGACAT |
| Internal bar code: | GCTCCTATCCCTGGGCATGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 84 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGCCAGAAGAGGTCACGC |
| Suggested primer 2: | CGCTGCCCTTACTTTTGAAG |