| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.162483 |
| Chromosome: | chromosome 3 |
| Location: | 6733908 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197850 | (1 of 2) PF11267 - Domain of unknown function (DUF3067) (DUF3067) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTAAGTGTTACCGCACATGCCGTTCTCT |
| Internal bar code: | GTAGGCCGGTAACCGGGAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1232 |
| LEAP-Seq percent confirming: | 86.728 |
| LEAP-Seq n confirming: | 660 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCGCTCTAGGATGCATGT |
| Suggested primer 2: | AGGCAGAGTGCCTGATGAGT |