| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.162511 |
| Chromosome: | chromosome 3 |
| Location: | 4221821 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174076 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAAAAGACACCAGAGGAATGCCGCTGCG |
| Internal bar code: | TCCGACCGGAGTCACTTTCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 95.7821 |
| LEAP-Seq n confirming: | 545 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGAGTTCAACGGTGCTT |
| Suggested primer 2: | ACAGTGTTCCTGCCAGCTCT |