| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162527 |
| Chromosome: | chromosome 6 |
| Location: | 3136284 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g275300 | (1 of 3) PF05670 - Domain of unknown function (DUF814) (DUF814) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATGGCATGACATGGGGGTTGCTTGACTA |
| Internal bar code: | CGGTCCGGGTCATGACGGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 99.5956 |
| LEAP-Seq n confirming: | 1970 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCACACTAAGGAGGCAGA |
| Suggested primer 2: | GTACACCTCCTTCTCTGCCG |