| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162558 |
| Chromosome: | chromosome 7 |
| Location: | 6370128 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g357550 | (1 of 3) K06236 - collagen, type I/II/III/V/XI/XXIV/XXVII, alpha (COL1AS) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATACCGGACCGGAGCAGAGCAGGAGGAG |
| Internal bar code: | AAAACGAGACGGCACGGGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 738 |
| LEAP-Seq percent confirming: | 99.8296 |
| LEAP-Seq n confirming: | 2343 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGTACCACACAGGGCCAC |
| Suggested primer 2: | AGTCCATGTCCAACGACTCC |